J Med Life Sci > Volume 10(2); 2013 > Article
Journal of Medicine and Life Science 2013;10(2):116-119.
DOI: https://doi.org/10.22730/jmls.2013.10.2.116    Published online December 18, 2018.
진귤(Citrus sunki) 과피추출물이 H4IIE 간세포 포도당합성에 미치는 영향
Effect of Extract of Citrus Sunki Peel on Gluconeogenesis in H4IIE Hepatoma Cells
Young-Ki Lee, Deok-Bae Park
Correspondence:  Deok-Bae Park,
Peel of Citrus sunki traditionally has a potential anti-inflammatory activity and traditionally has been used to alleviate respiratory symptoms in Jeju, Korea. However, little is known about the metabolic function of Citrus sunki peel. The present study investigated whether the extract of the fermented Citrus sunki peel might affect glucose production (gluconeogenesis) in H4IIE rat hepatoma cells. When H4IIE cells were incubated in glucose-free medium overnight, concentration of glucose in culture medium were elevated and treatment with ethanol extract of Citrus sunki peel (SE) significantly suppressed the glucose production. Protein levels of phosphoenolpyruvate carboxykinase (PEPCK) were also decreased by treatment with SE. Hesperidine and rutin, flavonoids present in SE, however, failed to suppress the glucose production. Addition of hydrogen peroxi dedecreased the viability of H4IIE cells. SE, hesperidin, rutin protected H4IIE cells against the cytotoxicity induced by hydrogen peroxide. In summary, SE can suppress glucose production in glucose-deficient conditions and protect cells against oxidative stress. It should be further studied which compounds are responsible for the gluconeogenic activity of SE.
Key Words: Citrus sunki, glucose production, H4IIE hepatoma cells

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