| 전기생리학적 연구를 위한 흰쥐의 해마절편조직 배양에서 Sindbis-virus
감염특성을 이용한 특정 단백질의 발현조절 기법 소개 |
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| Introduction of the Method to Regulate Protein Expression Using Sindbis-viral Infection in
Organotypic Hippocampal Slice Cultures of Rats for Electrophysiological Studies. |
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Kyeong-Deok Kim, Sung-Cherl Jung |
Correspondence:
Sung-Cherl Jung,
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| Abstract |
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There are several in-vitro preparations of brain tissue for electrophysiological researches. In this paper, we introduce an
organotypic slice culture preparation and its efficiency for viral infection to manipulate the expression of specific proteins in
hippocampal neurons. Using Sindbis virus is very useful for electrophysiological studies because of its high infection efficiency
with channel proteins in hippocampal neurons. Furthermore, the organotypic slice culture of hippocampus of rodents exhibits a
great benefit in both optical images and electrophysiological recordings as they are clearly thinner than 100 μm and show
hyper-connectivity of synapses. In the present paper, we discussed about the effective combination of organotypic slice culture
and viral infection with rat hippocampi, and in last, introduced some technical know-how to enhance the infection rate. This
method helps investigators to easily modify target proteins in neurons of mammalian central nervous system. |
| Key Words:
Electrophysiology, Hippocampus, Sindbis virus, GFP |
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