| 영양막 세포의 세포 증식력 측정 모델의 확립 |
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| Establishment of Assay Model for the Trophoblast Cell-line Proliferation |
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Soon-Sup Shim |
Correspondence:
Soon-Sup Shim,
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| Abstract |
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Preeclampsia is one of the major causes of pregnancy-related mortality and morbidity. While the pathophysiology is not well
examined, it is known that impeded early placental formation may be one etiologic step. The cytotoxic effect of lymphocytes on
the trophoblast may be one of immunological explanations. This study was designed to find out the optimal condition of cell
concentration and to establish an experimental model of cytotoxic effect of lymphocytes on the trophoblast cell-line proliferation.
In various conditions of cell concentration, the interaction between trophoblast cell-line and lymphocytes were measured by
CCK-8 kit, which assesses cell proliferation by absortiometry. The optimal condition (trophoblast cell-line: 105 cells/mL,
lymphocyte: 106 cells/mL) was selected for this model. We established a model for assessing the interaction between the
trophoblast cell-line and lymphocytes by measuring the cell proliferation. |
| Key Words:
Preeclampsia, Experimental Model, Trophoblast Cell-line, Lymphocytes, Cell Proliferation |
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